Heterogeneity of the Filamentous Haemagglutinin of Bordetella pertussis Studied with Monoclonal Antibodies
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چکیده
منابع مشابه
Mechanism of association of adenylate cyclase toxin with the surface of Bordetella pertussis: a role for toxin-filamentous haemagglutinin interaction.
Adenylate cyclase (AC) toxin from Bordetella pertussis is unusual in that, unlike most other members of the repeats-in-toxin family that are released into the extracellular milieu, it remains associated with the bacterial surface. In this study, we investigated the nature of the association of this toxin with the surface of B. pertussis. AC toxin was extracted from crude outer membrane preparat...
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Although substantial advancements have been made in the development of efficacious acellular vaccines against Bordetella pertussis, continued progress requires better understanding of the antigenic makeup of B. pertussis virulence factors, including filamentous hemagglutinin (FHA). To identify antigenic regions of FHA, phage display libraries constructed by using random fragments of the 10-kbp ...
متن کاملFimbrial typing of Bordetella pertussis isolates: agglutination with polyclonal and monoclonal antibodies.
On the basis of serotyping 62 strains of Bordetella pertussis, Guiso and colleagues (N. Guiso, C. H. Wirsing von König, C. Becker, and H. Hallander, Letter, J. Clin. Microbiol. 39:1684– 1685, 2001) concluded that monoclonal and polyclonal reagents showed good concordance but that the source of the monoclonal antibodies needed to be specified. We have conducted a similar study on 209 strains of ...
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Background and Aims: Pertussis is a highly contagious, vaccine-preventable disease. Determination of the seroepidemiology of pertussis makes possible the evaluation of pertussis immunity in a population. In this study, we determined the seroprevalence of Bordetella pertussis IgG antibodies in different age groups in Tehran, Iran. Materials and Methods: Overa...
متن کاملComparison of type 2 and type 6 fimbriae of Bordetella pertussis by using agglutinating monoclonal antibodies.
Two types of fimbriae have been identified on the pathogenic gram-negative organism Bordetella pertussis. Monoclonal antibodies to these fimbriae were produced to better understand the role of fimbriae as serotype-specific agglutinogens and to investigate the antigenic relationship between these fimbriae. Three monoclonal antibodies were identified that specifically agglutinated B. pertussis ce...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1983
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-129-9-2769